Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: The level of CD200 in follicular fluid and serum of patients who underwent IVF procedures. Correlations between CD200 concentration and the number of oocytes retrieved in FF (A) and serum (C) ( n = 43). The levels of CD200 in follicular fluid (B) and serum (D) were compared between the groups with higher (≥18, n = 12), middle (6–17, n = 23), and lower (≤5, n = 8) oocytes retrieved. The correlations between CD200 concentration and the level of E2 and P were analyzed (E) . ∗∗∗ P < 0.001. Abbreviations: E2, estradiol; P, progesterone.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: Effects of hCG on CD200 and inflammatory response pathway in KGN cells and the effects of CM on vascular permeability in HUVECs. The cells were treated with vehicle control (Ctrl) and 1, 10, and 100 IU/mL hCG for 24 h. The protein levels of CD200 and CD200R (A) , Cyp19a1, IL-1β, and TNFα (B) , and of NF-κB, p-NF-κB, p38 MAPK, and p-p38 MAPK (C) were examined. HUVECs were treated with condition culture medium from KGN for 24 h. (D) The levels of vasoactive proteins VEGF and VEGFR2, and cell junction proteins of Occludin, Claudin 5, and ZO-1 in HUVECs. (E) Fluorescent photomicrographs of HUVEC monolayer stained with TRITC-phalloidin with a final concentration of 20 mg/ml. Data were presented as means ± SD, * P < 0.5, ** P < 0.01, *** P < 0.001 versus Ctrl.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Permeability, Control, Staining, Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: Effects of CD200Fc on inflammatory response pathway in KGN cells and vascular permeability-related protein and cell cytoskeleton in HUVECs. (A) The cells were treated with vehicle (Ctrl), and 10 IU/ml hCG with or without 100 ng/ml CD200Fc for 24 h, and the protein levels of CD200, CD200R, Cyp19a1, IL-1β, and TNFα were examined by western blotting. (B) The ovarian expression of NF-κB, p-NF-κB, p38 MAPK, and p-p38 MAPK, and the relative NF-κB and p38 MAPK activity were derived as p-NF-κB and p-p38 MAPK, and are expressed as fold changes over Ctrl. HUVECs were treated with condition culture medium from KGN (10 IU/ml hCG with or without 100 ng/ml CD200Fc) for 24 h. (C) The levels of vasoactive proteins VEGF and VEGFR2, and cell junction proteins Occludin, Claudin 5, and ZO-1 were examined by western blotting. (D) Fluorescent photomicrographs of HUVECs monolayer stained with TRITC-phalloidin with a final concentration of 20 mg/ml. Data were presented as means ± SD, * P < 0.5, ** P < 0.01, *** P < 0.001 versus Ctrl.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Permeability, Western Blot, Expressing, Activity Assay, Derivative Assay, Staining, Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: The number of follicles at different stages, expression of CD200 and CD200R in EA-treated OHSS model. (A) The number of follicles at different stages in each group. (B) The ratio of corpora lutea/total follicles. (C) The protein expression of CD200 and CD200R in ovary of OHSS rats with or without EA treatment. (D) The CD200 levels in the serum of experimental groups were detected by ELISA. (E) The distribution of CD200 was detected by IHC, original magnification 40×, scale bar = 100 μm. Data were shown as means ± SD, * P < 0.5, ** P < 0.01, *** P < 0.001 versus OHSS group.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay